The Liver Regeneration and Tissue Engineering Group is seeking a highly motivated Research Technician to work on a collaborative study focused on the human fetal liver tissue microenvironment. You will have the opportunity to work in a multi-disciplinary environment, collaborating with other research groups at the Institute. We are looking for an organised, flexible, motivated scientist to play a key part in this exciting project.
Who we are looking for:
We require a minimum of a BSC or equivalent in biological science or related disciplines. You will work on laboratory-based studies investigating the microenvironment of the human fetal liver, how it changes during development and in particular the interaction between haematopoietic stem cells and fetal liver parenchymal and non-parenchymal cells and extracellular matrix proteins, for the identification of important signalling molecules for hematopoietic stem cell expansion in vitro. You should have experience in the main laboratory techniques we will be using: handling of human fetal liver samples, isolation and culture of different cell types from human fetal liver tissue, culture of human haematopoietic stem cells and CFU assays, preparation of matrix-enriched tissue samples (decellularisation) and their characterisation, 3D culture of fetal liver engineered constructs, flow cytometry and ELISA assays, gene expression analysis, Luminex Multiplex assay, immunohistochemistry and immunofluorescence and microscopy.