You will work collaboratively with other scientists in the Malaspina lab to develop novel experimental strategies to characterise mononuclear cells secretome and identify molecular targets released by cells in culture before and after treatment of novel therapeutics.
You will be trained and participate in research projects centered around the use of techniques like Olink and single molecular array (Simoa). You will be involved in a range of cell culturing techniques enabling the development and maintenance of existing induced pluripotent stem cells (IPSc) lines from blood mononuclear cells. This will include the derivation and cryopreservation of IPSc from fibroblasts and peripheral blood mononuclear cells (PBMC), the recovery of media to study the cells’ secretome and protocol modification to allow the best condition for proteomic and immunodetection analysis. You will also develop protocols of flow-cytometry and Mass Cytoff to characterize cells populations and sort target cell subtypes, be expected to interact with academic and industry partners, and work closely and under the supervision of the U2EM postdoctoral researchers at the QS MND Centre.
The role is available immediately and funded by the Reta Lila Weston Trust for one year in the first instance.